Coding

Part:BBa_K2486001:Design

Designed by: Fabio Nunes de Mello   Group: iGEM17_USP-Brazil   (2017-10-01)


His-tagged (EPIP)4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to add a His-tag and a HlyA secretion tag to the part. The base sequence was already codon optimized and did not contain any illegal restriction sites.


Source

Acquired from IDT gBlock synthesis. The sequence was originally created from rabbit anti-enolase antibody and was obtained from:

Wang, S., Ghosh, A., Bongio, N., Stebbings, K., Lampe, D. and Jacobs-Lorena, M. (2012). Fighting malaria with engineered symbiotic bacteria from vector mosquitoes. Proceedings of the National Academy of Sciences, 109(31), pp.12734-12739.

References

Wang, S., Ghosh, A., Bongio, N., Stebbings, K., Lampe, D. and Jacobs-Lorena, M. (2012). Fighting malaria with engineered symbiotic bacteria from vector mosquitoes. Proceedings of the National Academy of Sciences, 109(31), pp.12734-12739.

Ghosh, A., Coppens, I., Gardsvoll, H., Ploug, M. and Jacobs-Lorena, M. (2011). Plasmodium ookinetes coopt mammalian plasminogen to invade the mosquito midgut. Proceedings of the National Academy of Sciences, 108(41), pp.17153-17158.